UTILIZAÇÃO DE MÉTODOS MAIS SUSTENTÁVEIS PARA A EXTRAÇÃO DE QUITINA DE LARVAS DA MOSCA-SOLDADO-NEGRA
DOI:
https://doi.org/10.56238/revgeov17n5-088Palavras-chave:
Matriz, Larvas, Hermetia illucens L, Extração, PolissacarídeoResumo
As principais fontes do polissacarídeo quitina são as carapaças de crustáceos geradas como resíduos das atividades pesqueiras. Entretanto, a extração de quitina por essa metodologia é um processo altamente caro e poluente. Com o objetivo de minimizar o uso de agentes químicos, o presente trabalho teve como principal objetivo buscar novas matrizes para a extração de quitina, que permitam metodologias utilizando agentes químicos ambientalmente sustentáveis. A matriz escolhida foi a larva de Hermetia illucens L., e o processo de extração do polissacarídeo foi desenvolvido levando em consideração a propriedade física da quitina de ser solúvel em sistema aquoso aquecido. A metodologia consistiu inicialmente na extração do óleo das larvas de H. illucens por meio de maceração com diclorometano e, posteriormente, as larvas foram submetidas à extração a quente em autoclave com pressão e temperatura controladas, utilizando água como solvente. O extrato aquoso foi resfriado e adicionou-se etanol frio, o que favoreceu a precipitação do polissacarídeo. O polissacarídeo foi avaliado por FTIR e RMN e, pela comparação dos dados com a literatura, concluiu-se que a metodologia proposta foi eficiente para extrair γ-quitina.
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