SCREENING OF ENVIRONMENTAL YEASTS FROM THE CERRADO FOR THE PRODUCTION OF HYDROLYTIC ENZYMES OF BIOTECHNOLOGICAL INTEREST
DOI:
https://doi.org/10.56238/revgeov17n4-047Keywords:
Biotechnology, Biodiversity, Enzymes, YeastsAbstract
Environmental yeasts represent an underexplored source of novel hydrolytic enzymes with biotechnological potential. The objective of this study was to evaluate the potential for hydrolytic enzyme production by environmental yeasts from the Cerrado. The tested yeasts (18 isolates) were derived from lobeira (Solanum lycocarpum) fruits, cashew fruits and pseudofruits (Anacardium occidentale), pequi (Caryocar coriaceum) fruits, the body surface of Spodoptera frugiperda (Lepidoptera: Noctuidae), and corn kernels (Zea mays). The isolates were grown and/or reactivated on Potato Dextrose Agar (PDA) medium at 25 ± 3 °C for 48 h. Screening for the production of amylases, cellulases, pectinases, and proteases on solid medium was performed, and enzyme activity indices (EAI) were calculated. Of the total number of isolates tested (18), 44.4% demonstrated cellulolytic activity, 27.8% amylolytic activity, and 22.2% pectinolytic activity. None demonstrated proteolytic activity. Isolates LC2, LC1, and P2L1 demonstrated activity for three of the four enzymes tested (cellulase, amylase, and pectinase). Isolate LC2 exhibited the highest EAI values, achieving the best EAI for cellulolytic activity under acidic pH conditions. It was concluded that yeasts with biotechnological potential, such as isolates LC2, LC1, and P2L1, can be obtained from environmental substrates, especially edible fruits and pseudofruits. Studies such as this reinforce the importance of investing in the conservation and enhancement of Cerrado biodiversity.
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