DETERMINATION OF FIBER IN AN AUTOCLAVE UNDER DIFFERENT CONDITIONS
DOI:
https://doi.org/10.56238/revgeov17n5-049Keywords:
Concentrate Feeds, NDF, ADF, Filter Bag, Nonwoven FabricAbstract
The objective of this study was to evaluate the effects of different autoclave temperature and time conditions on neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents determined using a nonwoven fabric filter bag (100 g/m²). Four commercial concentrate feedstuffs were evaluated: corn meal, soybean meal, wheat bran, and sorghum grain. NDF and ADF analyses were performed in an autoclave under three operational conditions: 105 °C for 60 min, 110 °C for 40 min, and 120 °C for 40 min. A completely randomized design was adopted. Data were subjected to analysis of variance, and treatment means were compared using Tukey’s test at the 5% significance level. Increasing autoclave temperature affected fiber analysis results, causing significant reductions in NDF and ADF contents for some concentrate feedstuffs, especially under the 120 °C condition. Soybean meal showed the greatest reduction in NDF values as temperature increased, whereas wheat bran exhibited greater stability under the evaluated thermal conditions. For ADF, sorghum grain showed progressive reductions in values as temperature increased. The results indicate that more severe operational conditions may promote greater solubilization of cell wall components, reducing the recovery of fibrous fractions during the analytical procedure. Therefore, autoclave temperature and time conditions directly influence NDF and ADF determinations, reinforcing the importance of methodological standardization to ensure greater precision, repeatability, and comparability in laboratory fiber analyses used in animal nutrition.
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